Procoagulant Activity of Lymphocyte - Macrophage Populations in Rabbits : Selective Increases in Marrow , Blood , and Spleen Cells

The present experiments examine leukocyte procoagulant activity using mononuclear cell populations purified or enriched from rabbit bone marrow. blood. spleen. lymph node. thymus, and pulmonary alveoli. Cells from these six sites. obtained from control and endotoxemic animals and assayed without an intermediate culture step. were found to have procoagulant activity identified as tissue factor. Under control conditions. tissue factor activity was found to be at low levels in marrow and blood populations compared to median activities 3and 1 1 -fold higher in populations from spleen and lymph node, and 33and 45-fold higher in thymus and alveolar populations. By contrast to respective controls. significantly increased amounts of tissue factor (35-. 1 5-, and 1 2-fold at median levels) were found in marrow, blood, and spleen popula-